Bikaner railway station is at an elevation of 226 metres (741 ft) and was assigned the code – BKN.

Bikaner is served by more than 21 pairs of trains each day with multiple daily connections to Delhi, Ahmedabad, Vadodara, Surat, Mumbai and Chandigarh. Within Rajasthan, there are also multiple daily trains available for Jodhpur, Jaipur, Nagaur, Ratangarh, Churu, Barmer and Jaisalamer. There are also daily trains to Kota.

Daily connections to Pathankot, Amritsar, Jammu, Kalka, Haridwar, Agra, Kanpur, Lucknow, Allahabad, Varanasi, Patna, Durgapur, Kolkata, Guwahati and Dibrugarh are also available.

Bikaner railway station was constructed in 1891 with a Rs. 3,46,000 donation by Rai Bahadur Dewan Bahadur Sir Kasturchand Daga, a known trader from the city.

In 1889, the two states of Jodhpur and Bikaner formed the Jodhpur–Bikaner Railway to promote railway development jointly within the Rajputana Agency. In 1891 the 1,000 mm (3 ft 3 ³⁄₈ in)-wide metre-gauge Jodhpur–Bikaner line was opened. In 1901–02, the metre-gauge line was extended to Bathinda